Introductory flow cytometry lectures
Seminar presentation on "How to communicate Science to the Public" (Purdue University, September 24, 2009) NEW!!
Microscopy Series - Below are the new updated versions of the class BMS 524 "Introduction to Confocal Microscopy and Image Analysis" that have been available since 1994. Many are updated in 2007. PLEASE EMAIL ME WITH ANY ERRORS YOU FIND IN THESE SLIDES !!!
TIPS and FIXES for POWERPOINT _ how to embed FLASH, Fixes for SOUND FILE problems etc
JPR=Dr. J. Paul Robinson, Purdue University Cytometry Laboratories; BR=Dr. Bartek Rajwa, PUCL; SK=Steve Kelley PUCL
Flow Cytometry Series (Used in my Graduate class BMS 631 - (versions these slides have been made publicly available since 1991. J. Paul Robinson, Professor, Purdue University Cytometry Laboratories. They are based on Howard Shaprio's great text "Practical Flow Cytometry" and many have page numbers to reference the material in that text.
These lectures are updated more frequently on the regular class page at this page
These lectures are undated, but many have been here for many years, so they may not be as up updated as you might like...
IF YOU USE OUR SLIDES....PLEASE GIVE PROPER CREDIT
Many of these slides have been published in several of our Purdue Cytometry CDROM series, and may be used without copyright restriction except for commercial or for profit use, PROVIDED YOU GIVE CREDIT TO THE AUTHOR, and reference this site.
Want to just listen to 2 Introductory flow cytometry lectures?? Online flow cytometry lectures -E-lectures created in 1999-2000
How to Give a Seminar : "Presentation 101 for Graduate Students" This presentation is a guide for how to present a good seminar. It discusses the important aspects of a good presentation. It is a great start for graduate/postgraduate students.Seminar presentation on "How to communicate Science to the Public" (Purdue University, September 24, 2009) NEW!!
Microscopy Series - Below are the new updated versions of the class BMS 524 "Introduction to Confocal Microscopy and Image Analysis" that have been available since 1994. Many are updated in 2007. PLEASE EMAIL ME WITH ANY ERRORS YOU FIND IN THESE SLIDES !!!
TIPS and FIXES for POWERPOINT _ how to embed FLASH, Fixes for SOUND FILE problems etc
Lecture | Title | Prof | Size | PPT: or Video View |
GRAD STUDENTS.... "Presentation 101 for Graduate Students" | JPR | Some help preparing your seminars... | ||
IF YOU USE OUR SLIDES....PLEASE GIVE PROPER CREDIT | ||||
1 | The Principles of Microscopy Part 1 [49 slides] | JPR | 6.153 Meg | PPT |
2 | The Principles of Microscopy Part 2 [41 slides] | JPR | 2.714 Meg | PPT |
3 | Image Formats and Image Manipulations [29 slides] | SK | 5.794 Meg | PPT--- |
4 | The Principles of Microscopy Part 3 [31 slides] | JPR | 4.265 Meg | PPT---VIEW the video lecture |
5 | Fluorescence and Fluorescent Probes [52 slides] | JPR | 7.46 Meg | PPT:::: VIEW the video lecture |
6 | The Principles of Confocal Microscopy [44 slides] | BR | 5.022 Meg | PPT---VIEW the video lecture |
7 | 3D Imaging, Rotations and Stereo Imaging [33 slides] | JPR | 3.163 Meg | PPT |
8 | Principles of 2D image analysis - Morphometry (I) [46 slides] | JPR | 1.623 Meg | PPT---VIEW the video lecture |
9 | Applications of Morphometry (II) [36 slides] | JPR | 9.237 Meg | PPT---VIEW the video lecture |
10 | Live Cell Imaging Application in Confocal Microscopy [38 slides] | JPR | 9.545 Meg | PPT |
11 | Applications of Confocal Microscopy [24 slides] | JPR | 2.349 Meg | PPT |
12 | Sample Preparation of Confocal Microscopy [25 slides] | JPR | 8.4 Meg | PPT--- VIEW the video lecture |
13 | Additional Applications of Confocal Microscopy [25 slides] | BR | ||
IF YOU USE OUR SLIDES....PLEASE GIVE PROPER CREDIT |
Flow Cytometry Series (Used in my Graduate class BMS 631 - (versions these slides have been made publicly available since 1991. J. Paul Robinson, Professor, Purdue University Cytometry Laboratories. They are based on Howard Shaprio's great text "Practical Flow Cytometry" and many have page numbers to reference the material in that text.
These lectures are updated more frequently on the regular class page at this page
A | Introductory flow cytometry: Part 1- E-lecture - automated online lecture | JPR | Listen | 63 Minutes |
B | Introductory flow cytometry: Part 2- E-lecture - automated online lecture | JPR | Listen | 60 minutes |
1 | Fluorescence Quantitation - An overview of the salient issues including QC | JPR | 15.4 Megs | PPT |
2 | BMS 631 - LECTURE 1 Flow Cytometry: Theory (2006) | JPR | 28.8 Megs | PPT |
3 | BMS 631 Flow Cytometry: Theory - Lecture 2 Whos and whys of flow cytometry - History(2006) | JPR | 8.32 Megs | PPT |
4 | BMS 602A/631 - Lecture 3: : Light and Matter (2006) | JPR | 0.68 Megs | PPT |
5 | BMS 602A/631: Lecture 4 : Light and Fluorescence (2005) | JPR | 0.51 Megs | PPT |
6 | BMS 631 – Lecture 5: Properties and Applications of Light Sources (2005) | JPR | 3.34 Megs | PPT |
7 | BMS 631 - Lecture 6: : Optical Systems (2005) | JPR | 24.53 Megs | PPT |
8 | BMS 631 - Lecture 7: Optics - Filter Properties & manipulation of light in flow cytometry (2005) | JPR | 0.17 Megs | PPT |
9 | BMS 631: Lecture 8: Detectors (2004) | JPR | 2.8 megs | PPT |
10 | BMS 631 - Lecture: Flow systems and hydrodynamics (2005) | JPR | 8.41 Megs | PPT |
11 | BMS 631 - Lecture 10: Multiparameter data analysis (2004) | JPR | 1.71 Megs | PPT |
IF YOU USE OUR SLIDES....PLEASE GIVE PROPER CREDIT |
These lectures are undated, but many have been here for many years, so they may not be as up updated as you might like...
- Introduction to Flow Cytometry - 1 28 Slides by Dr. J. Paul Robinson Download the PPT file 5.3 Megs
- Introduction to Flow Cytometry -2 32 Slides by Dr. J. Paul Robinson Download the PPT file 1.05 Megs
- Flow Cytometry Lecture 3 - Light and Matter 28 Slides by Dr. J. Paul Robinson Download the PPT file 213 K
- Flow Cytometry lecture 4 - Optical Systems 29 Slides by Dr. J. Paul Robinson Download the PPT file 1.94 Megs
- Flow Cytometry Lecture 5 - Light Sources and Optical Systems 26 Slides by Dr. J. Paul Robinson Download the PPT file 3.9 Megs
- Flow Cytometry Lecture 6 - Optics: Filter Properties 41 Slides by Dr. J. Paul Robinson Download the PPT file 235 K
- Flow Cytometry Lecture 7 - Detectors & Fluidics 54 Slides by Dr. J. Paul Robinson Download the PPT file 9.7 Megs
- Flow cytometry lecture 8 - Electronic measurements & Signal Processing 24 Slides by Dr. J. Paul Robinson Download the PPT file 95 K
- Flow Cytometry Lecture 9 - Data Collection: Linear, Log, Ratios... 24 Slides by Dr. J. Paul Robinson Download hte PPT file 292 K
IF YOU USE OUR SLIDES....PLEASE GIVE PROPER CREDIT
- Basic flow cytometry theory [17 slides] Dr. J. Paul Robinson, Purdue University Cytometry Laboratories
- DNA analysis [28 slides] Dr. J. Paul Robinson, Purdue University Cytometry Laboratories
- Multiple Color Analysis [3 slides] Dr. William Telford, Flow Lab at Hospital for Special Surgery, New York, NY
- Light Scatter [2 slides] Dr. William Telford, Flow Lab at Hospital for Special Surgery, New York, NY
- Excitation / Emission peaks[9 slides] Dr. William Telford, Flow Lab at Hospital for Special Surgery, New York, NY
- Applications [14 slides] Dr. J. Paul Robinson, Purdue University Cytometry Laboratories
- Antibody Binding [49 slides] Dr. Carleton Stewart, (Retired)
- Multiparameter Immunofluorescence [67 slides] Dr. Carleton C. Stewart, Ph.D. & Sigrid J. Stewart, (Retired)
- Compensation [16 slides] Dr. Carleton C. Stewart, Ph.D. & Sigrid J. Stewart, (Retired)
- Simultaneous Measurement of Necrotic, Apoptotic Dead and Live Cells [4 slides] Dr. Carleton Stewart, (Retired)
- Immunophenotyping Leukemias & Lymphomas [23 slides] Dr. Carleton Stewart, (Retired)
- Breast Cancer: DNA Content & Relevant Markers [4 slides] Dr. Carleton Stewart, (retired)
- Automated Reticulocyte Analysis [27 slides] B. H. Davis
- Immunophenotyping of Hematopoietic Progenitor Cells by Multiparameter Flow Cytometry [20 slides] Dr. Carleton Stewart, (retired)
- IF YOU USE OUR SLIDES....PLEASE GIVE PROPER CREDIT
All of the above material has been stolen from my website. If you want to use my lectures, there are clear restictions on their use,but they certainly cannot be uploaded to a website like this - the person/company that has uploaded my materials is plain and simply a thief. I share my materials under certain conditions, but I sure don't approve of scumbags who post my lectures on their sites (like this) trying to make money. If you want to use my lectures at least have the decency to go to my own website...cyto.purdue.edu
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